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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene is noticed in several tumors, which leads to chromosome instability, and therefore may contribute to tumorigenesis. This protein was observed to affiliate with, and become linked to, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a significant role in advertising cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have already been explained. Two added splice variants ended up described but in depth nucleotide sequence info is just not yet readily available.
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Blocking peptides are peptides that bind exclusively for the goal antibody and block antibody binding. These peptide ordinarily includes the epitope recognized with the antibody.
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This manual outlines further more attainable optimization of mobile and immunoassay parameters to make certain the very best effects are...
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This guidebook outlines even further probable optimization of mobile and immunoassay parameters to make sure the very best effects are...
Antibodies bound to the blocking peptide no more bind towards the epitope within the focus on protein. By evaluating the staining through the blocked antibody vs . the antibody on your own, one can see which staining is unique.
Concentrate on Details Cyclin E belongs to the remarkably conserved cyclin loved ones, whose associates are characterised by a remarkable periodicity in protein abundance from the cell cycle. Cyclins purpose as regulators of CDK kinases. Diverse cyclins exhibit distinctive expression and degradation designs which add to your temporal coordination of each mitotic party. Cyclin E types a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S changeover. This protein accumulates within the G1-S phase boundary and is also degraded as cells progress through S section.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by equally antibodies...
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